cholesterol biosynthesis pathway
SynName: Anti -FDFT1, CT (FDFT1, Squalene synthase, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase); FDFT1, CT (FDFT1, Squalene synthase, FPP:FPP farnesyltransferase, Farnesyl-diphosphate farnesyltransferase); *Specificity: HumanImmunogen: FDFT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the C-terminal region of human FDFT1.; *Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.; *Accession#: CAG33314.1; Q6IAX1; *Description: This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene.; *NCBI Summary: This gene encodes a membrane-associated enzyme located at a branch point in the mevalonate pathway. The encoded protein is the first specific enzyme in cholesterol biosynthesis, catalyzing the dimerization of two molecules of farnesyl diphosphate in a two-step reaction to form squalene. [provided by RefSeq, Jul 2008]
*Specificity: This assay has high sensitivity and excellent specificity for detection of Human CYP7A1. No significant cross-reactivity or interference between Human CYP7A1 and analogues was observed.Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10% Three samples of known concentration were tested in twenty assays to assess.; *Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.; *Accession#: NP_000771.2; NM_000780.3; P22680; *Description: Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP7A bound in the initial step. The color development is stopped and the intensity of the color is measured.; *NCBI Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the pr...
Questions & answers
Explore: Association of high cholesterol levels with nerve spoil
Lipid profiles. METHODS: This about enrolled 136 patients with MS (n = 272 eyes. A subset of 88 patients received criterion reversal visual-evoked potential (PRVEP) testing
. 45% had a curriculum vitae of optic neuritis (ON). Subjects received optical coherence tomography (OCT) testing to assess RNFL thickness and visual acuity testing with Snellen charts.
Google news feed
Schematic representation of the cholesterol biosynthesis pathway, with ...
www.biomedcentral.com - Figure
Several hydroxysterol compounds formed from cholesterolby ...